Anti-UBE3A Monoclonal Antibody

Product Name

Anti-UBE3A Monoclonal Antibody

View all E6AP/UBE3A Antibodies

SKU/Catalog Number

M00582

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-UBE3A Monoclonal Antibody catalog # M00582. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-UBE3A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00582)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADBA-21

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human UBE3A E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00582 is reactive to UBE3A in Human, Mouse, Rat

Applications

M00582 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

47 kDa

Calculated molecular weight

100.688kDa

Background of E6AP/UBE3A

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000

Validation Images & Assay Conditions

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Western blot analysis of UBE3A expression in K562 cell lysate.

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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

Gene/Protein Information For UBE3A (Source: Uniprot.org, NCBI)

Alternative Names

ANCR; ANCREPVE6AP; AS; CTCL tumor antigen se37-2; E6AP ubiquitin-protein ligase; E6AP; E6-AP; EC 6.3.2.-; EPVE6AP; FLJ26981; HPVE6A; human papilloma virus E6-associated protein; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54; UBE3A; ubiquitin protein ligase E3A; ubiquitin-protein ligase E3A UBE3A ANCR, AS, E6-AP, EPVE6AP, HPVE6A ubiquitin protein ligase E3A ubiquitin-protein ligase E3A|CTCL tumor antigen se37-2|E6AP ubiquitin-protein ligase|HECT-type ubiquitin transferase E3A|human papilloma virus E6-associated protein|human papillomavirus E6-associated protein|oncogenic protein-associated protein E6-AP|renal carcinoma antigen NY-REN-54

For more info on UBE3A, check out the UBE3A Infographic

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In this infographic, you will see the following information for UBE3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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